The painting represents Maddona with her child, Saint Anna and San Giuseppe.
They are inserted into an urban landscape that could be an idealized Jerusalem or a view of the walls of Siena.
The painting is of very good pictorial quality and is stylistically typical of the Tuscan Magnierissmo.
Unfortunately, the lower left corner is missing.
It comes from Tuscany, probably Siena.
The painting has never been restored and is in its original condition.
When cleaning the paint, much stronger colors will come out.